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10x genomics cellranger pipeline ![]() 10x Genomics Cellranger Pipeline, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/algorithms+cell+ranger+single+cell+software+suite+10x+genomics+version+6+1+2/10x+cellranger+genomics+pipeline/pmc10014726-211-16-16 Average 86 stars, based on 1 article reviews
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Image Search Results
Journal: Frontiers in Immunology
Article Title: Strategies for optimizing CITE-seq for human islets and other tissues
doi: 10.3389/fimmu.2023.1107582
Figure Lengend Snippet: Workflow and combined islet and spleen lineage maps. (A) Example workflow of CITE-seq. Spleen and islet samples were enriched for CD45 + immune cells. Immune cells from spleen were stained with oligo-antibody conjugated TotalSeqC antibodies and sent for 10x sequencing. Islet immune cells were either were stained with oligo-antibody conjugated TotalSeqC antibodies or stained for both oligo-antibody conjugated TotalSeqC and flow cytometry antibodies at the same time and FACS sorted to enrich for NK cells and ILCs and then sent for 10x sequencing. (B) UMAPs of islet cell types, islet cell origin (FACS sorted or CD45-enriched), and spleen cell types. (C) Merged islet and spleen lineage, cell types and tissue of origin UMAPs. (D) Composition of cell types from each organ. (E) T, NK and ILC lineage markers. (F) Myeloid lineage markers. (G) B cell, plasma cell and mast cell lineage markers.
Article Snippet: Read alignment to the reference human genome (GRCh38/hg38) and gene expression matrices were generated by the
Techniques: Staining, Sequencing, Flow Cytometry, Clinical Proteomics